Genetic complementation analysis in Pycnoporus cinnabarinus

White-rot basidiomycetes are known as very powerful degraders of wood components. The relative enzyme activities during the degrading process may vary between different species

Parallelization using a BDD-C of a multiscale strategy with non lineal localization based on a NKS

En trabajos previos la localización no lineal fue presentada y estudiada para casos de grandes desplazamientos pero solo para problemas de estructuras con respuestas globales estables y resueltos de manera secuencial. En este trabajo se presenta la paralelización de esta estrategia de cálculo mediante el método Balancing Domain Decomposition by Constrains (BDD-C). Finalmente, se realiza una validación de la implementación en elasticidad lineal, mediante la comprobación del speed-up y la extensibilidad numérica.In previous works, the nonlinear localization was first presented and studied in the case of large displacements but only for globally stable structural responses. In this paper, the parallelization of this computational strategy using a Balancing Domain Decomposition by Constrains (BDD-C) is presented. Finally, a validation of this implementation is carried out in lineal elasticity, verifying the speed-up and the numerical extensibility.Peer Reviewe

Screening for lignin peroxidase genes in natural isolates of white rot fungi

To find lingin peroxidase genes similar to those of Phanerochaete chrysosporium, we have studied several species of white rot fungi collected in nature. The methodology has been DNA hybridization techniques using two synthetic oligonucleotides with a sequence that corresponds to a fragment of the H8 lignin peroxidase gene from P. chrysosporium (Schalch et al. 1989. Mol. Cell. Biol. 9:2743-2747) controlling the structure of the predominant form of the enzyme in this fungus

Presence of double stranded RNA in natural isolates of Pycnoporus cinnabarinus

While studying the nucleic acids of different strains of P. cinnabarinus (Pc58, Pc470.3, Pc470.6), the presence of dsRNA molecules (1.7 kb to 3.6 kb) was detected in two of them

A method for the preparation of intact chromosomal DNA of Pycnoporus cinnabarinus

We describe an improved procedure to obtain chromosomal DNA of Pycnoporus cinnabarinus, which was used in pulse field gel electrophoresis employing a contour-clamped homogenous electric field (CHEF). Four bands of chromosomal DNA were separated. The procedure may be useful to study the genome of other filamentous fungi

Contrasting patterns of selection between MHC I and II across populations of Humboldt and Magellanic penguins

Indexación: Web of ScienceThe evolutionary and adaptive potential of populations or species facing an emerging infectious disease depends on their genetic diversity in genes, such as the major histocompatibility complex (MHC). In birds, MHC class I deals predominantly with intracellular infections (e.g., viruses) and MHC class II with extracellular infections (e.g., bacteria). Therefore, patterns of MHC I and II diversity may differ between species and across populations of species depending on the relative effect of local and global environmental selective pressures, genetic drift, and gene flow. We hypothesize that high gene flow among populations of Humboldt and Magellanic penguins limits local adaptation in MHC I and MHC II, and signatures of selection differ between markers, locations, and species. We evaluated the MHC I and II diversity using 454 next-generation sequencing of 100 Humboldt and 75 Magellanic penguins from seven different breeding colonies. Higher genetic diversity was observed in MHC I than MHC II for both species, explained by more than one MHC I loci identified. Large population sizes, high gene flow, and/or similar selection pressures maintain diversity but limit local adaptation in MHC I. A pattern of isolation by distance was observed for MHC II for Humboldt penguin suggesting local adaptation, mainly on the northernmost studied locality. Furthermore, trans species alleles were found due to a recent speciation for the genus or convergent evolution. High MHC I and MHC II gene diversity described is extremely advantageous for the long term survival of the species.http://onlinelibrary.wiley.com/doi/10.1002/ece3.2502/epd

Nominal Exchange Rate in Chile: Predictions based on technical analysis

This work presents a review of the main indicators used in the technical analysis of the peso-dollar parity. We explain the usual interpretations of these indicators and we also explore the ability that the Relative Strength Index (RSI) may have to predict exchange rate returns at daily frequency. The predictive exercises are carried out using both in-sample and out-of-sample analyses. Our results show a robust ability of the RSI to predict nominal exchange rate returns for horizons under seven weeks.

Damage Analysis of Reinforced Concrete Structures with Substandard Detailing

The goal of this study is to investigate seismic behaviour of existing R/C buildings designed and constructed in accordance with standards that do not meet current seismic code requirements. In these structures, not only flexure, but also shear and bond-slip deformation mechanisms need to be considered, both separately and in combination. To serve this goal, a finite element model is developed for inelastic seismic analysis of complete planar R/C frames. The proposed finite element is able to capture gradual spread of inelastic flexural and shear deformations as well as their interaction in the end regions of R/C members. Additionally, it is capable of predicting shear failures caused by degradation of shear strength in the plastic hinges of R/C elements, as well as pullout failures caused by inadequate anchorage of the reinforcement in the joint regions. The finite element is fully implemented in the general inelastic finite element code IDARC2D and it is verified against experimental results involving individual column and plane frame specimens with nonductile detailing. It is shown that, in all cases, satisfactory correlation is established between the model predictions and the experimental evidence. Finally, parametric studies are conducted to illustrate the significance of each deformation mechanism on the seismic response of the specimens under investigation. It is concluded, that all deformation mechanisms, as well as their interaction, should be taken into consideration in order to predict reliably seismic damage of R/C structures with substandard detailing